HuagongsheHuagongshe Chemistry Print Report · For research reference only
Formate → 1,3-Propanediol
Participants
| Role | Name | HCID | Charge |
|---|---|---|---|
| Reactant | Formate | HCID 283 | — |
| Reactant | Lactate | HCID 91435 | — |
| Reactant | (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal | HCID 107526 | — |
| Reactant | HCID 178418840 | HCID 178418840 | — |
| Product | 1,3-Propanediol | HCID 10442 | — |
| Product | (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal | HCID 107526 | — |
Reaction Steps
The plasmid pTPG(−) and the control plasmid pTLH1 were introduced into the E. coli NZN 111 (pfl :cat, Idh :kan) strain, a strain incapable of producing formate and lactate in anaerobiosis. These two recombinant strains were then grown anaerobically for 48 hours in the LB medium+glucose+10 μg/ml of tetracycline without regulation of pH. The results presented in Table 5 show that E. coli can produce significant quantities of 1,3-propanediol from glucose after transformation by the plasmid pTPG.
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patent number | US07695943B2 |
| Source dataset | uspto-grants-2010_04 |
| ORD ID | ord-836d6c633ed54cceae662041055e1974 |
| Source note | uspto-grants-2010_04 |