Formate → 1,3-Propanediol

HRID 641123Notes

Reaction equation

HRID 641123 reaction equation
C([O-])=O.C([O-])(=O)C(C)O.[O:10]=[CH:11][C@@H:12]([C@H:14]([C@@H:16]([C@@H:18]([CH2:20][OH:21])[OH:19])[OH:17])[OH:15])[OH:13].C[C@]1(O)[C@@H]2C(=C(O)[C@]3(O)C(=O)C(C(N)=O)=C(O)[C@@H](N(C)C)[C@@H]3C2)C(=O)C2C(O)=CC=CC1=2>>[CH2:14]([OH:15])[CH2:12][CH2:11][OH:10].[O:10]=[CH:11][C@@H:12]([C@H:14]([C@@H:16]([C@@H:18]([CH2:20][OH:21])[OH:19])[OH:17])[OH:15])[OH:13]

Participants

6

Reactant 4

HCID 283FormateHCID 91435LactateHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanalHCID 178418840HCID 178418840, compound

Product 2

HCID 104421,3-PropanediolHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal

Procedure

The plasmid pTPG(−) and the control plasmid pTLH1 were introduced into the E. coli NZN 111 (pfl :cat, Idh :kan) strain, a strain incapable of producing formate and lactate in anaerobiosis. These two recombinant strains were then grown anaerobically for 48 hours in the LB medium+glucose+10 μg/ml of tetracycline without regulation of pH. The results presented in Table 5 show that E. coli can produce significant quantities of 1,3-propanediol from glucose after transformation by the plasmid pTPG.

Workup

  1. additionThe plasmid pTPG(−) and the control plasmid pTLH1 were introduced into the E

Sources & evidence

ORD record
ord-836d6c633ed54cceae662041055e1974
Source dataset
uspto-grants-2010_04
Patent number
US07695943B2

Reaction notes

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