反应详情

温度

37 °C

27 mL Alcalase 2.4 L FG (Novozymes A/S; DK 2880 Bagsvaerd) were added to a solution of 38.0 g (213.5 mmol) disodium hydrogen phosphate dihydrate in 500 mL water at a temperature of 37° C. and the pH was adjusted to 7.5 by the addition of sodium dihydrogen phosphate dihydrate. Then at 37° C. with stirring 29.7 g (101.9 mmol) ethyl 2-acetylamino-3-(3,4-diethyl-phenyl)-propionate dissolved in 150 mL acetone was added dropwise. The pH value of the reaction mixture was kept constantly kept in the range from pH 7.4 to pH 7.6 by the addition of 1 M NaOH. After the addition had ended the mixture was stirred for 4 h at 37° C. The reaction mixture was extracted three times with tert-butylmethylether, the combined organic extracts were washed with 15% K2CO3 solution, dried and evaporated down under reduced pressure.

1. additionwas added dropwise
2. additionAfter the addition
3. extractionThe reaction mixture was extracted three times with tert-butylmethylether
4. washthe combined organic extracts were washed with 15% K2CO3 solution
5. customdried
6. customevaporated down under reduced pressure