反应详情

Methanol (40 ml), tetrahydrofuran (20 ml) and acetic acid (0.3 ml) were added to 4-nitrobenzyl 7-[2-(tert-butoxycarbonylmethoxyimino)-2-(2-formamidothiazol-4-yl)acetamido]-3-chloro-3-cephem-4-carboxylate (syn isomer, 3.8 g). 10% Palladium carbon (1.9 g) containing water (3 ml) was added to the solution, and subjected to catalytic reduction for 4 hours. After removal of palladium carbon by filtration, the filtrate was concentrated in vacuo. To the residue were added ethyl acetate and water, and the solution was adjusted to pH 7.5 with a saturated solution of sodium bicarbonate. After the aqueous layer was separated, ethyl acetate was added to the aqueous layer, and adjusted to pH 2.0 with 10% hydrochloric acid. The ethyl acetate layer was separated. The organic solution was washed with a saturated solution of sodium chloride, dried over magnesium sulfate and concentrated in vacuo. The residue was triturated with diisopropyl ether, and then the precipitates were collected by filtration and dried to give 7-[2-(tert-butoxycarbonylmethoxyimino)-2-(2-formamidothiazol- 4-yl)acetamido]-3-chloro-3-cephem-4-carboxylic acid (syn isomer, 2.31 g).

1. additionwas added to the solution
2. customAfter removal of palladium carbon
3. filtrationby filtration
4. concentrationthe filtrate was concentrated in vacuo
5. additionTo the residue were added ethyl acetate and water
6. customAfter the aqueous layer was separated
7. additionethyl acetate was added to the aqueous layer
8. customThe ethyl acetate layer was separated
9. washThe organic solution was washed with a saturated solution of sodium chloride
10. dry with materialdried over magnesium sulfate
11. concentrationconcentrated in vacuo
12. customThe residue was triturated with diisopropyl ether
13. filtrationthe precipitates were collected by filtration
14. customdried