反应详情

To 30 ml of an ethyl acetate solution of compound (4) prepared from 1.52 g of compound (3) by the method shown in Reference example 2 was added 785 mg of (S)-N-cyclohexyl-2-pyrrolidinecarboxamide (36) prepared by a known method and then the reaction was maintained overnight in a refrigerator. The reaction mixture was concentrated and the residue thus formed was subjected to silica gel column chromatography. By eluting the product with chloroform-methanol-aqueous ammonia (90:10:1), 1.30 g of Nα -benzyloxycarbonyl-L-histidyl-N-cyclohexyl-L-prolinamide (37) was obtained.

1. customprepared by a known method
2. temperaturethe reaction was maintained overnight in a refrigerator
3. concentrationThe reaction mixture was concentrated
4. customthe residue thus formed
5. washBy eluting the product with chloroform-methanol-aqueous ammonia (90:10:1)