反应详情

To 45 ml of an ethyl acetate solution of Nα -benzyloxycarbonyl-L-histidine azide (4) prepared from 3.03 g of Nα -benzyloxycarbonyl-L-histidine hydrazide (3) by a known method was added 1.52 g of compound (94) under ice-cooling and the reaction was maintained overnight in a refrigerator. The reaction mixture was concentrated and the residue thus formed was subjected to silica gel column chromatography. By eluting the product with chloroform-methanol (95:5), 2.49 g of Nα -benzyloxycarbonyl-L-histidyl-N-phenyl-L-prolinamide (95) was obtained.

1. temperaturecooling
2. temperaturethe reaction was maintained overnight in a refrigerator
3. concentrationThe reaction mixture was concentrated
4. customthe residue thus formed
5. washBy eluting the product with chloroform-methanol (95:5)