Citric Acid → 12-Hydroxyicosa-5,8,10,14-tetraenoic acid

HRID 653531Notes

Reaction equation

HRID 653531 reaction equation
[CH3:1][CH2:2][CH2:3][CH2:4][CH2:5][CH:6](O)/[CH:7]=[CH:8]/[CH:9]=[CH:10]\[CH2:11]/[CH:12]=[CH:13]\[CH2:14]/[CH:15]=[CH:16]\[CH2:17][CH2:18][CH2:19][C:20]([OH:22])=[O:21].C(O)(=O)CC(CC(O)=O)(C(O)=O)[OH:27]>>[CH3:1][CH2:2][CH2:3][CH2:4][CH2:5][CH:6]=[CH:7][CH2:8][CH:9]([OH:27])[CH:10]=[CH:11][CH:12]=[CH:13][CH2:14][CH:15]=[CH:16][CH2:17][CH2:18][CH2:19][C:20]([OH:22])=[O:21]

Participants

3

Reactant 2

HCID 311Citric AcidHCID 9966861(5Z,8Z,11Z,13E)-15-Hydroxyicosa-5,8,11,13-tetraenoic acid2×

Product 1

HCID 141312-Hydroxyicosa-5,8,10,14-tetraenoic acid

Procedure

Unlabeled 15-HETE and (3H)-12-HETE are added to the citric acid quenched samples as internal standards. After chromatography of the extracted products on silica gel, (14C)-12-HETE is located by the UV absorbence of the added 15-HETE (with which it cochromatographs) and is quantitated by scintillation spectroscopy.

Sources & evidence

ORD record
ord-5e3c259638694dc4b4227083222ada72
Source dataset
uspto-grants-1987_06
Patent number
US04677210

Reaction notes

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