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HuagongsheHuagongshe Chemistry Print Report · For research reference only
HRID 653531Generated: October 30, 2026

Citric Acid → 12-Hydroxyicosa-5,8,10,14-tetraenoic acid

https://huagongshe.com/reaction/653531

Reaction equation

Participants

RoleNameHCIDCharge
ReactantCitric AcidHCID 311—
Reactant(5Z,8Z,11Z,13E)-15-Hydroxyicosa-5,8,11,13-tetraenoic acidHCID 9966861—
Product12-Hydroxyicosa-5,8,10,14-tetraenoic acidHCID 1413—

Reaction Steps

Unlabeled 15-HETE and (3H)-12-HETE are added to the citric acid quenched samples as internal standards. After chromatography of the extracted products on silica gel, (14C)-12-HETE is located by the UV absorbence of the added 15-HETE (with which it cochromatographs) and is quantitated by scintillation spectroscopy.

Provenance & Evidence

DOI10.6084/m9.figshare.5104873.v1
Patent numberUS04677210
Source datasetuspto-grants-1987_06
ORD IDord-5e3c259638694dc4b4227083222ada72
Source noteuspto-grants-1987_06