反应详情

To a solution of (E)-3-(2-quinolinyl)-2-propenoic acid (2) (0.4 g, 2.0 mmol) in dry dimethylformamide (3 ml), at ice bath temperature (˜0° C.), under an atmosphere of argon, N,N′-carbonyldiimidazole (0.65 g, 4.0 mmol) was added over 15 minutes and the reaction mixture was stirred at this temperature for 2 hours. Triethylamine (0.85 ml, 6.0 mmol), followed by hydroxylamine hydrochloride (0.42 g, 6.0 mmol), were added to the reaction, and the obtained mixture was stirred at ice bath temperature for 1 hour, and then at room temperature overnight. The reaction mixture was poured into water (30 ml), acidified with 1 N HCl to pH 5 and extracted with ethyl acetate (3×40 ml). The combined extracts were washed with water (50 ml), brine (20 ml), and dried (Na2SO4). The solvent was evaporated and the residue was chromatographed on silica gel with acetonitrile-water (20:1) as eluent to give the title compound as white crystals. M.p. 159.5-160.5° C. 1H NMR (DMSO-d6, HMDSO), δ: 7.12 (1H, d, J=16.0 Hz); 7.49-8.18 (6H, m); 8.44 (1H, d, J=8.0 Hz); 10.13 (2H, br s). HPLC analysis on Symmetry C8 column: impurities 2.0% (column size 3.9×150 mm; mobile phase acetonitrile-0.1M phosphate buffer, pH 2.5, 20:80; detector UV 254 nm; flow rate 1.2 ml/min; sample concentration 0.5 mg/ml). Anal. Calcd for C12H10N2O2*H2O: C, 62.06; H, 5.21; N, 12.06. Found, %: C, 61.95; H, 4.91; N, 12.07.

1. additionwere added to the reaction
2. stirringthe obtained mixture was stirred at ice bath temperature for 1 hour
3. customat room temperature
4. customovernight
5. extractionextracted with ethyl acetate (3×40 ml)
6. washThe combined extracts were washed with water (50 ml), brine (20 ml)
7. dry with materialdried (Na2SO4)
8. customThe solvent was evaporated
9. customthe residue was chromatographed on silica gel with acetonitrile-water (20:1) as eluent