Hydrochloric Acid → HCID 67174106, compound

A solution of tert-butyl ((2S)-6-nitro-2′-oxo-1′-{[2-(trimethylsilyl)ethoxy]methyl}-1,1′,2′,3-tetrahydrospiro[indene-2,3′-pyrrolo[2,3-b]pyridin]-5-yl)acetate from Step C (2.01 g, 3.83 mmol) in MeOH (25 mL) was saturated with HCl (g) and stood at ambient temperature for 18 h. The mixture was concentrated to dryness in vacuo, then redissolved in MeOH (25 mL). This stirred solution was adjusted to pH 10 with 10 N NaOH and ethylene diamine (0.26 mL, 3.83 mmol) was added. The resulting mixture was stirred for 3 h, then concentrated to dryness in vacuo. The residue was dissolved in THF (25 mL) and 1 N NaOH (25 mL, 25 mmol) was added. The mixture was stirred at ambient temperature for 18 h, then the THF was removed under reduced pressure. The residual mixture was partitioned between saturated aqueous NaHCO3 (50 mL) and EtOAc (100 mL). The organic layer was discarded and the aqueous layer was adjusted to pH 2 with aqueous HCl, then extracted with EtOAc (3×200 mL). The combined organic layers were dried over Na2 SO4, filtered, and concentrated under reduced pressure to give the title compound. MS: m/z=340 (M+1).

1. concentrationThe mixture was concentrated to dryness in vacuo
2. dissolutionredissolved in MeOH (25 mL)
3. additionwas added
4. concentrationconcentrated to dryness in vacuo
5. dissolutionThe residue was dissolved in THF (25 mL)
6. stirringThe mixture was stirred at ambient temperature for 18 h
7. customthe THF was removed under reduced pressure
8. customThe residual mixture was partitioned between saturated aqueous NaHCO3 (50 mL) and EtOAc (100 mL)
9. extractionextracted with EtOAc (3×200 mL)
10. customThe combined organic layers were dried over Na2 SO4
11. filtrationfiltered
12. concentrationconcentrated under reduced pressure

ORD record

ord-5978ec9ace4542758e88559b5a690091

Source dataset

uspto-grants-2011_12

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US08071772B2