反应详情

Each strain of the microbial cells thus obtained was recovered from the agar media, and suspended in a 0.1 M borate buffer (pH 9.0) containing 10 mM EDTA so as to prepare a suspension containing 100 g/l of wet microbial cells. 0.1 ml of each microbial cell suspension was admixed with 0.1 ml of 100 mM borate buffer (pH 9.0) containing 10 mM EDTA, 200 mM L-alanine methyl ester hydrochloride and 400 mM L-glutamine to prepare a total volume 0.2 ml of the reaction mixture, which was then subjected to the reaction at 20° C. for the time period shown in Table 1. The amount (mM) of L-alanyl-L-glutamine (Ala-Gin) produced by each reaction is shown in Table 1.

1. customEach strain of the microbial cells thus obtained
2. customwas recovered from the agar media
3. additioncontaining 10 mM EDTA so as
4. customto prepare a suspension
5. additioncontaining 100 g/l of wet microbial cells
6. customto prepare a total volume 0.2 ml of the reaction mixture, which
7. customwas then subjected to the reaction at 20° C. for the time period