L-Glutamine → L-Alanyl-L-Glutamine

Participants

Reaction Steps

Each strain of the microbial cells thus obtained was recovered from the agar media, and suspended in a 0.1 M borate buffer (pH 9.0) containing 10 mM EDTA so as to prepare a suspension containing 100 g/l of wet microbial cells. 0.1 ml of each microbial cell suspension was admixed with 0.1 ml of 100 mM borate buffer (pH 9.0) containing 10 mM EDTA, 200 mM L-alanine methyl ester hydrochloride and 400 mM L-glutamine to prepare a total volume 0.2 ml of the reaction mixture, which was then subjected to the reaction at 20° C. for the time period shown in Table 1. The amount (mM) of L-alanyl-L-glutamine (Ala-Gin) produced by each reaction is shown in Table 1.

Provenance & Evidence