反应详情

A mixture of 2-guanidino-4-[2-(2-cyano-3-methylguanidino)ethylthiomethyl]thiazole (1.0 g.), and 2.5 N hydrochloric acid (12 ml.) was heated for four minutes on the steam bath. On cooling a white solid precipitated which was applied to Merck 60 F-254 preparative thin layer chromatography plates and eluted with chloroform/methanol/ammonia (s.g. 0.880) 7:3:0.5 v/v/v. The yellow foam obtained was dissolved in methanol and methanolic hydrogen chloride added. The solution was evaporated to dryness and the residue crystallized from methanol to give 2-guanidino-4-[2-(2-carbamoyl-3-methylguanidino)ethylthiomethyl]thiazole hydrochloride, m.p. 148°-150° C.

1. temperaturewas heated for four minutes on the steam bath
2. temperatureOn cooling a white solid
3. customprecipitated which
4. washeluted with chloroform/methanol/ammonia (s.g. 0.880) 7:3:0.5 v/v/v
5. customThe yellow foam obtained
6. customThe solution was evaporated to dryness
7. customthe residue crystallized from methanol