反应详情

4 M HCl in 1,4-dioxane (4 mL, 16 mmol) was added to a solution of tert-butyl 4-(5-(benzyl carbamoyl)-4-methyl oxazol-2-yl)-3-((pyridin-3-yloxy)methyl)piperazine-1-carboxylate in MeOH (1 mL). After 1 h, the reaction mixture was concentrated under reduced pressure and purified by HPLC (10 to 90% MeCN/0.1% TFA in H2O/0.1% TFA gradient). The desired chromatography fractions were concentrated under reduced pressure, dissolved in MeOH (1 mL), and treated with 4 M HCl in 1,4-dioxane. This was concentrated under reduced pressure, yielding 3.6 mg (3%) of the title compound as an off-white solid. LC-MS: RT=4.12 min, [M+H]+=408.7.

1. concentrationthe reaction mixture was concentrated under reduced pressure
2. custompurified by HPLC (10 to 90% MeCN/0.1% TFA in H2O/0.1% TFA gradient)
3. concentrationThe desired chromatography fractions were concentrated under reduced pressure
4. dissolutiondissolved in MeOH (1 mL)
5. additiontreated with 4 M HCl in 1,4-dioxane
6. concentrationThis was concentrated under reduced pressure