Sodium Chloride → HCID 178454332, compound

HRID 719856Notes

Reaction equation

HRID 719856 reaction equation
[CH3:1][C:2]([CH3:25])([O:4][C:5]([N:7]([C:18]([O:20][C:21]([CH3:24])([CH3:23])[CH3:22])=[O:19])[C:8]1[N:9]=[CH:10][C:11]([C:14](=[NH:17])[NH:15][OH:16])=[N:12][CH:13]=1)=[O:6])[CH3:3].Cl[C:27]([O:29][CH2:30][CH:31]1[C:43]2[CH:42]=[CH:41][CH:40]=[CH:39][C:38]=2[C:37]2[C:32]1=[CH:33][CH:34]=[CH:35][CH:36]=2)=[O:28].[Cl-].[Na+]>N1C=CC=CC=1.C(OCC)(=O)C.O>[CH3:22][C:21]([CH3:24])([O:20][C:18]([N:7]([C:5]([O:4][C:2]([CH3:25])([CH3:1])[CH3:3])=[O:6])[C:8]1[N:9]=[CH:10][C:11]([C:14](=[NH:17])[NH:15][O:16][C:27]([O:29][CH2:30][CH:31]2[C:32]3[CH:33]=[CH:34][CH:35]=[CH:36][C:37]=3[C:38]3[C:43]2=[CH:42][CH:41]=[CH:40][CH:39]=3)=[O:28])=[N:12][CH:13]=1)=[O:19])[CH3:23]

Participants

7

Reactant 3

HCID 5234Sodium ChlorideHCID 343679-Fluorenylmethyl chloroformateHCID 178454331HCID 178454331, compound

Product 1

HCID 178454332HCID 178454332, compound

Reagents, catalysts & solvents 3

HCID 962WaterSolventHCID 1049PyridineSolventHCID 8857Ethyl AcetateSolvent

Conditions

Temperature
25 °C

Procedure

A solution of the 5-[[bis[(1,1-dimethylethoxy)carbonyl]]amino]-N-hydroxy-2-pyrazinecarboximidamide (77.0 mg, 0.218 mmol) in pyridine (2 mL) at 25° C. was treated with 9-fluorenylmethyl chloroformate (68.0 mg, 0.263 mmol). The reaction mixture was stirred at 25° C. for 45 min. The reaction mixture was then diluted with ethyl acetate, water, and a saturated aqueous sodium chloride solution. The mixture was shaken and separated. The aqueous layer was extracted with ethyl acetate. The combined organic layers were dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo. Biotage chromatography (FLASH 40S, Silica, 1/9 to 1/1 ethyl acetate/hexanes) afforded the desired 5-[[bis[(1,1-dimethylethoxy)carbonyl]]amino]-N-[[[(9H-fluoren-9-yl)methoxy]carbonyl]oxy]-2-pyrazine carboximidamide. This material was dissolved in methylene chloride (0.5 mL). The solution was cooled to 0° C. and then was treated with trifluoroacetic acid (0.16 mL, 2.077 mmol). The reaction mixture was stirred at 0° C. for 30 min and at 25° C. for 30 min. The reaction mixture was then treated with additional trifluoroacetic acid (1.0 mL, 12.99 mmol) and stirred at 25° C. for 2 h. The reaction mixture was further treated with trifluoroacetic acid (1.0 mL, 12.99 mmol) and stirred at 25° C. for 2 h. The reaction mixture was then diluted with methylene chloride, washed three times with a saturated aqueous sodium bicarbonate solution, dried over anhydrous sodium sulfate, filtered, concentrated in vacuo, and then dried under high vacuum to afford 5-amino-N-[[[(9H-fluoren-9-yl)methoxy]carbonyl]oxy]-2-pyrazinecarboximidamide (36.7 mg, 44.9%) as a white solid: LRMS for C20H17N5O3 (M+H)+ at m/z=376

Workup

  1. stirringThe mixture was shaken
  2. customseparated
  3. extractionThe aqueous layer was extracted with ethyl acetate
  4. dry with materialThe combined organic layers were dried over anhydrous sodium sulfate
  5. filtrationfiltered
  6. concentrationconcentrated in vacuo

Sources & evidence

ORD record
ord-3bc77c1dde97414692b4ca9433b7caa7
Source dataset
uspto-grants-2006_11
Patent number
US07132425B2

Reaction notes

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