反应详情

温度

0 °C

The solution of 250 mg (0.237 mmol) of Boc-Ala-Arg(Tos)-Pro-Ala-Lys(ClZ)OBzl (SEQ ID NO: 13), 1 ml of anisole and 2 ml of HF was stirred at 0° C. for 2 h. The reaction mixture was evaporated under reduced pressure to remove HF. To the residue 2 ml of HF were added and the solution was stirred at 0° C. for another 1 h. The reaction mixture was evaporated under reduced pressure to remove HF. The residue was triturated with ether and the resulted solid was purified on the Sephadex G-10 column. The collected fractions were lyophilized to provide 100 mg (77.8%) of the title compound. Mp. 224–6° C., FAB-MS (m/e) 542[M+H]+.

1. customThe reaction mixture was evaporated under reduced pressure
2. customto remove HF
3. additionTo the residue 2 ml of HF were added
4. customThe reaction mixture was evaporated under reduced pressure
5. customto remove HF
6. customThe residue was triturated with ether
7. customthe resulted solid was purified on the Sephadex G-10 column