DL-alanine → L-Lysine

HRID 724775Notes

Reaction equation

HRID 724775 reaction equation
O=C[C@@H]([C@H]([C@@H]([C@@H](CO)O)O)O)O.S([O-])([O-])(=O)=O.[NH4+].[NH4+].[K].S([O-])([O-])(=O)=O.[Mg+2].CC1[N+](CC2C=NC(C)=NC=2N)=CSC=1CCO.Cl.[Cl-].[OH:47][C:48](CCCC[C@H]1[C@@H]2[C@@H](NC(N2)=O)CS1)=[O:49].[C:63]([NH2:71])(=O)[C:64]1[CH:69]=[CH:68][CH:67]=[N:66]C=1.NC(C(O)=O)C>>[NH2:71][C@H:63]([C:48]([OH:49])=[O:47])[CH2:64][CH2:69][CH2:68][CH2:67][NH2:66]

Participants

9

Reactant 8

HCID 602DL-alanineHCID 936NicotinamideHCID 6202Thiamine HydrochlorideHCID 24083Sulfuric acid magnesium salt (1:1)HCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanalHCID 171548BiotinHCID 5462222PotassiumHCID 6097028Ammonium Sulfate

Product 1

HCID 5962L-Lysine

Procedure

An aqueous solution medium having a composition of 10% of glucose, 2% of ammonium sulfate, 0.1% of potassium primary phosphate, 0.04% of magnesium sulfate, 0.001% of ferrous sulfate, 200 μg/l of thiamine hydrochloride, 500 μg/l of biotin, 5 ml/dl of Aji-Eki®, 1 mg/dl of nicotinamide and 0.1% of DL-alanine, at pH 7.0, was charged in an amount of 300 ml into separate small sized glass jar fermenters. After sterilizing in a conventional manner, the various L-lysine-producing bacterial strains shown in Table 11, which had been previously grown in bouillon slants at 30° C. for 48 hours, were inoculated thereon. Then, incubation was carried out at 31.5° C. for 48 hours at 1200 rpm at an aeration rate of 1/2 volume per minute, while keeping the pH at 7.0 by the addition of ammonia gas. After completion of the fermentation, the yield of L-lysine produced and accumulated in the solution based on glucose was determined and is shown in Table 11.

Workup

  1. additionat pH 7.0, was charged in an amount of 300 ml
  2. custominto separate small sized glass jar fermenters

Sources & evidence

ORD record
ord-fcc6bdbb49234b849d680b9220aaf263
Source dataset
uspto-grants-1998_01
Patent number
US05705370

Reaction notes

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