Phosphoric Acid → (2-Hydroxy-1,1-bis(hydroxymethyl)ethyl)ammonium dihydrogen phosphate

Participants

Reaction Steps

A sample of well agitated fermentation mixture (5.0 ml) is centrifuged for five minutes at 17,000 rpm in a centrifuge tube, the supernate decanted and discarded, and the pellet resuspended in 4.9 ml of 0.1M tris phosphate buffer. The tris phosphate buffer is prepared by dissolving 12.1 grams of Sigma Chem. Co. T-1503 Trizma base in 800 ml of deionized water, adjusting the pH at 25° C. to 7.5 with phosphoric acid and diluting with deionized water to a final volume of 1000 ml.

Provenance & Evidence