Phosphoric Acid → (2-Hydroxy-1,1-bis(hydroxymethyl)ethyl)ammonium dihydrogen phosphate

HRID 727766Notes

Reaction equation

HRID 727766 reaction equation
[CH2:1]([OH:8])[C:2]([NH2:7])([CH2:5][OH:6])[CH2:3][OH:4].[P:9](=[O:13])([OH:12])([OH:11])[OH:10]>O>[CH2:1]([OH:8])[C:2]([NH2:7])([CH2:5][OH:6])[CH2:3][OH:4].[OH:11][P:9]([OH:13])([OH:12])=[O:10]

Participants

4

Reactant 2

HCID 1004Phosphoric AcidHCID 6503Tris(hydroxymethyl)aminomethane

Product 1

HCID 3083992(2-Hydroxy-1,1-bis(hydroxymethyl)ethyl)ammonium dihydrogen phosphate

Reagents, catalysts & solvents 1

HCID 962WaterSolvent2×

Procedure

A sample of well agitated fermentation mixture (5.0 ml) is centrifuged for five minutes at 17,000 rpm in a centrifuge tube, the supernate decanted and discarded, and the pellet resuspended in 4.9 ml of 0.1M tris phosphate buffer. The tris phosphate buffer is prepared by dissolving 12.1 grams of Sigma Chem. Co. T-1503 Trizma base in 800 ml of deionized water, adjusting the pH at 25° C. to 7.5 with phosphoric acid and diluting with deionized water to a final volume of 1000 ml.

Workup

  1. customthe supernate decanted
  2. dissolutionby dissolving 12.1 grams of Sigma Chem
  3. customat 25° C.

Sources & evidence

ORD record
ord-5b9c7780e36b4c9391c0eaef9d762d50
Source dataset
uspto-grants-1991_09
Patent number
US05045465

Reaction notes

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