反应详情

1-tert-butyl-3-(4-nitrophenyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine (21 mg, 0.082 mmol) was dissolved in a solution of formic acid (2 mL) and conc. HCl (0.2 mL) and heated to reflux for 30 min. Reaction was concentrated in vacuo and purified by RP-HPLC (MeCN:H2O:0.1% TFA). ESI-MS (M+H)+ m/z calcd 257.1, found 257.3.

1. temperatureheated
2. temperatureto reflux for 30 min
3. customReaction
4. concentrationwas concentrated in vacuo
5. custompurified by RP-HPLC (MeCN:H2O:0.1% TFA)