Glycolic Acid → Formate

Participants

Reaction Steps

The procedure described in Example 1 was repeated using an aqueous solution containing glycolic acid (0.500M), diethyl aminomethylphosphonate (DEAMPA, 0.398M), FMN (0.01 mM), isobutyric acid (HPLC internal standard, 0.10M), spinach glycolate oxidase (1.0 IU/mL), and soluble Aspergillus niger catalase (14,000 IU/mL) at pH 8.3 and at 5° C. After 20 h, the HPLC yields of glyoxylate, formate, and oxalate were 97.4%, 0.8%, and 1.8%, respectively, and no glycolate remained. The remaining activities of glycolate oxidase and catalase were 10% and 95%, respectively, of their initial values.

Provenance & Evidence