Glycolic Acid → Formate

Participants

Reaction Steps

The reaction in Example 6 was repeated, using an aqueous solution containing glycolic acid (0.50M), DEAMPA (0.525M), FMN (0.01 mM), isobutyric acid (HPLC internal standard, 0.10M), spinach glycolate oxidase (1.0 IU/mL), and soluble Aspergillus niger catalase (1,400 IU/mL) at pH 8.3. After 23 h, the HPLC yields of glyoxylate, formate, and oxalate were 95.3%, 3.9%, and 1.5%, respectively, and no glycolate remained. The remaining activities of glycolate oxidase and catalase were 12% and 100%, respectively, of their initial values.

Provenance & Evidence