Glycolic Acid → Formate

HRID 743964Notes

Reaction equation

HRID 743964 reaction equation
[C:1]([OH:5])(=[O:4])[CH2:2][OH:3].[C:6]([OH:11])(=[O:10])C(C)C.[C:12]([O-:16])(=[O:15])[CH2:13][OH:14]>>[C:1]([O-:5])(=[O:4])[CH:2]=[O:3].[CH:6]([O-:11])=[O:10].[C:13]([O-:3])(=[O:14])[C:12]([O-:16])=[O:15]

Participants

6

Reactant 3

HCID 757Glycolic AcidHCID 6590Isobutyric AcidHCID 5460308Hydroxyacetate

Product 3

HCID 283FormateHCID 71081OxalateHCID 3614358Npc324351

Procedure

The reaction in Example 6 was repeated, using an aqueous solution containing glycolic acid (0.50M), DEAMPA (0.525M), FMN (0.01 mM), isobutyric acid (HPLC internal standard, 0.10M), spinach glycolate oxidase (1.0 IU/mL), and soluble Aspergillus niger catalase (1,400 IU/mL) at pH 8.3. After 23 h, the HPLC yields of glyoxylate, formate, and oxalate were 95.3%, 3.9%, and 1.5%, respectively, and no glycolate remained. The remaining activities of glycolate oxidase and catalase were 12% and 100%, respectively, of their initial values.

Workup

  1. customThe reaction in Example 6

Sources & evidence

ORD record
ord-9aac8bb3cec44e48b5d91e2bb6b903ea
Source dataset
uspto-grants-1996_09
Patent number
US05559020

Reaction notes

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