Glycine → Glutathione
Reaction equation
[NH2:1][C@H:2]([C:8]([OH:10])=[O:9])[CH2:3][CH2:4][C:5]([OH:7])=O.[NH2:11][C@H:12]([C:15]([OH:17])=O)[CH2:13][SH:14].[NH2:18][CH2:19][C:20]([OH:22])=[O:21].[Cl-].[Mg+2].[Cl-].C(OP([O-])([O-])=O)(=O)C.P(OC[C@H]1O[C@@H](N2C3N=CN=C(N)C=3N=C2)[C@H](O)[C@@H]1O)(OP(OP(O)(O)=O)(O)=O)(=O)O>>[NH2:1][C@@H:2]([CH2:3][CH2:4][C:5]([NH:11][C@H:12]([C:15]([NH:18][CH2:19][C:20]([OH:22])=[O:21])=[O:17])[CH2:13][SH:14])=[O:7])[C:8]([OH:10])=[O:9]Participants
Procedure
Strains shown in the following Table 2 were respectively cultured in a similar manner to that described in Example 3. After completion of the culturing, on each occasion, the microbial cells were collected by centrifugation (8,000 r.p.m./10 min.) of the cultured broth and were then subjected to ultrasonic treatment (90 KHz/5 min.) to prepare a cell-free extract. This cell-free extracted solution (0.05 ml) was added to a 50 mM tris buffer solution (9.95 ml; pH 7.5) containing L-glutamic acid (25 mM), L-cysteine (25 mM), glycine (50 mM), magnesium chloride (10 mM), acetylphosphate (10 mM) and ATP (5 mM) to effect the enzymatic reaction at 37° C. for one hour. After completion of the reaction, the amount of glutathione formed in the reaction solution was measured to determine the accumulated amount of glutathione per one mg of protein. The results are shown in the following table.
Workup
- customAfter completion of the culturing, on each occasion, the microbial cells were collected by centrifugation (8,000 r.p.m
- custom10 min.
- customwere then subjected to ultrasonic treatment (90 KHz/5 min.)
- customto prepare a cell-free extract
- customthe enzymatic reaction at 37° C. for one hour
- customAfter completion of the reaction
Sources & evidence
- ORD record
- ord-7a9895d8ba3f4703a6652254fe1bba6f
- Source dataset
- uspto-grants-1986_07
- Patent number
- US04598046
Reaction notes
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