Hydrochloric Acid → Acetate

HRID 745897Notes

Reaction equation

HRID 745897 reaction equation
[CH3:1][O:2][C:3](=[O:9])[C@H:4]([C@@H:6]([CH3:8])[OH:7])[NH2:5].O.Cl>C(O)(=O)C>[CH3:1][O:2][C:3](=[O:9])[C@H:4]([C@@H:6]([CH3:8])[OH:7])[NH2:5].[C:3]([O-:9])(=[O:2])[CH3:4]

Participants

6

Reactant 3

HCID 313Hydrochloric AcidHCID 962WaterHCID 2734894L-Threonine methyl ester

Product 2

HCID 175AcetateYield 90.2%HCID 2734894L-Threonine methyl ester

Reagents, catalysts & solvents 1

HCID 176Acetic AcidSolvent

Procedure

1.5 ml of a 5M solution of L-threonine methyl ester, acetate containing 200 mg of porcine insulin were prepared by dissolving 200 mg of porcine insulin in 750 μ liters of 10M acetic acid and adding 1 g of L-threonine methyl ester thereto. 2 mg of porcine trypsin were added, and the clear reaction mixture having a pH value of 6.3 was left at 20° C. The reaction was stopped after 90 hours by adding 15 ml of water and adjusting the pH value to 3 with 5N hydrochloric acid.

Workup

  1. addition2 mg of porcine trypsin were added
  2. waitwas left at 20° C

Sources & evidence

ORD record
ord-ad0c1e9526c24d0d9eae17aa01c053b2
Source dataset
uspto-grants-1986_07
Patent number
US04601979

Reaction notes

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