反应详情

Heat the mixture of 6-(propane-2-sulfonylamino)-4′-[3-(propane-2-sulfonylamino)-thiophen-2-yl]-biphenyl-2-carboxylic acid methyl ester (46 mg, 0.08 mmol) and LiOH (30 mg, 1.25 mmol) in 3 ml of THF/Methanol/water (3/2/1, v/v/v) to 60 C for 3 h. Remove solvent and dissolve in water (35 mL). Wash with methylene chloride (2×20 mL). Neutralize the aq. solution to a pH between 2 and 3. Further purification with flash chromatography affords the title compound. MS (m/e): 521.1(M−1).

1. temperatureHeat
2. washWash with methylene chloride (2×20 mL)
3. customFurther purification with flash chromatography