反应详情

温度

100 °C

A mixture of ethyl 2-benzyl-8-hydroxy-1-oxo-1,2,3,4-tetrahydropyrrolo-[1,2-α]pyrazine-7-carboxylate (0.30 g; see Example 1) and aqueous sodium hydroxide (1 M, 2 mL) in ethanol (20 mL) was heated in a sealed tube at 100° C. overnight. The product mixture was acidified with addition of TFA and concentrated under vacuum. The residue was subjected to HPLC purification on C-18 stationary phase eluted with water/acetonitrile/TFA mobile phase. Collection and lyophilization of appropriate fractions provided the title compound.

1. concentrationconcentrated under vacuum
2. customThe residue was subjected to HPLC purification on C-18 stationary phase
3. washeluted with water/acetonitrile/TFA mobile phase
4. customCollection and lyophilization of appropriate fractions