反应详情

Weigh into a round bottom flask 3-bromopyrazolo[1,5-a]pyrimidine (Lynch et al. Can. J. Chem. 1975, 53, 119-122; 0.095 g, 0.48 mmol), 2-(pyridin-2-yl)-5,6-dihydro-4H-pyrrolo[1,2-b]pyrazole-3-boronic acid (Preparation 5; 0.10 g, 0.44 mmol), triphenylphosphine (Aldrich; 0.007 g, 0.027 mmol) and tris(dibenzylideneacetone)dipalladium(0) (Aldrich; 0.015 g, 0.016 mmol). Add p-dioxane (6 ml) and 2.0M aqueous potassium carbonate (2 mL). Stir and reflux under nitrogen for 6 h. Dilute with ethyl acetate, separate the organic layer, concentrate under reduced pressure, and chromatograph on flash silica using neat acetonitrile. Purify on a preparative reverse phase C-18 high-performance chromatography column using a gradient from 5% to 100% acetonitrile in 0.03% aqueous hydrochloric acid. Concentrate the pure fractions under reduced pressure, dissolve the residue in distilled water, and make basic by adding 1.0M aqueous sodium hydroxide. Extract with dichloromethane, dry the organic layer over anhydrous sodium sulfate, filter, and concentrate under reduced pressure to obtain 6 mg (4.5%) of the title compound as a yellow solid. TOF MS ES+ exact mass calculated for C17H15N6 (M+1): m/z=303.1358; Found: 303.1371. 1H NMR (400 MHz, CDCl3) δ 8.66 (d, J=2 Hz, 1H), 8.64 (d, J=2 Hz, 1H), 8.41 (dd, J=4, 2 Hz, 1H), 8.31 (s, 1H), 7.70 (m, 2H), 7.21 (m, 1H), 6.79 (dd, J=7, 4 Hz, 1H), 4.30 (t, J=7 Hz, 2H), 3.10 (t, J=8 Hz, 2H), 2.68 (m, 2H).

1. temperaturereflux under nitrogen for 6 h
2. customseparate the organic layer
3. concentrationconcentrate under reduced pressure, and chromatograph on flash silica using neat acetonitrile
4. customPurify on a preparative reverse phase C-18 high-performance chromatography column
5. concentrationConcentrate the pure fractions under reduced pressure
6. dissolutiondissolve the residue in distilled water
7. additionby adding 1.0M aqueous sodium hydroxide
8. extractionExtract with dichloromethane
9. dry with materialdry the organic layer over anhydrous sodium sulfate
10. filtrationfilter
11. concentrationconcentrate under reduced pressure