HCID 178419017, compound → HCID 178511768, compound

HRID 839777Notes

Reaction equation

HRID 839777 reaction equation
C(NCC([NH:10][C@H:11]([C:19]([NH:21][CH2:22][CH2:23][C:24]1[N:28]=[CH:27][NH:26][CH:25]=1)=[O:20])[CH2:12][C:13]1[CH:18]=[CH:17][CH:16]=[CH:15][CH:14]=1)=O)(=O)C(C)=C.NCCC1N=CNC=1>>[NH2:10][C@H:11]([C:19]([NH:21][CH2:22][CH2:23][C:24]1[N:28]=[CH:27][NH:26][CH:25]=1)=[O:20])[CH2:12][C:13]1[CH:18]=[CH:17][CH:16]=[CH:15][CH:14]=1

Participants

3

Reactant 2

HCID 178419017HCID 178419017, compound2×HCID 178454537HCID 178454537, compound

Product 1

HCID 178511768HCID 178511768, compound

Procedure

N-(Phenylalanyl)histamine was prepared by the method described by H. Arold and L. Rietschel (Z. Chem. 1969, 9, 144) and used in the polymeranalogous reaction with the copolymer KoII described in Example 1. The copolymer (KoV) was obtained which contained 1.1% of N(N'-(methacryloyl)glycyl)-L-phenylalanylhistamine units. It was incubated with chymotrypsin by the method described in Example 1. The released histamine was determined according P. A. Shore, A. Burkhalter and V. H. Cohn, Jr. (J. Pharmacol. exp. theor. 127, 182, 1959). The initial concentration of the bonded histamine was 1×10-3M and the chymotrypsin concentration 0.5 mg/ml. The initial cleavage rate was constant for 80 minutes with the slope K = 1.2×10-6 mole min-1 mg-1. The limit cleavage was 28%.

Workup

  1. customRietschel (Z. Chem. 1969, 9, 144) and used in the polymeranalogous reaction with the copolymer KoII
  2. customThe copolymer (KoV) was obtained which

Sources & evidence

ORD record
ord-6fa013210dfa4faeb348582e26511c7e
Source dataset
uspto-grants-1978_06
Patent number
US04097470

Reaction notes

Loading…