HCID 178419017, compound → HCID 178511768, compound

Participants

Reaction Steps

N-(Phenylalanyl)histamine was prepared by the method described by H. Arold and L. Rietschel (Z. Chem. 1969, 9, 144) and used in the polymeranalogous reaction with the copolymer KoII described in Example 1. The copolymer (KoV) was obtained which contained 1.1% of N(N'-(methacryloyl)glycyl)-L-phenylalanylhistamine units. It was incubated with chymotrypsin by the method described in Example 1. The released histamine was determined according P. A. Shore, A. Burkhalter and V. H. Cohn, Jr. (J. Pharmacol. exp. theor. 127, 182, 1959). The initial concentration of the bonded histamine was 1×10-3M and the chymotrypsin concentration 0.5 mg/ml. The initial cleavage rate was constant for 80 minutes with the slope K = 1.2×10-6 mole min-1 mg-1. The limit cleavage was 28%.

Provenance & Evidence