反应详情

An estimated 30 nmol of the amino-derivatized 2-3 nm platinum cluster dissolved in 0.6 M triethylammonium bicarbonate in 20% isopropanol/water was evaporated to dryness five times from methanol, then dissolved in 3 mL of diclhoromethane, and treated with 200 nmol of palmitic anhydride. The mixture was stirred for one hour at room temperature, then washed three times with 0.1 M sodium phosphate buffer at pH 6.5. The remaining reaction mixture was evaporated to dryness, redissolved in 2:1 methanol/chloroform, then separated by gel filtration (GH25, Amicon eluted with 2:1 methanol:chloroform); the first species to elute is the dark brown palmitamido-platinum cluster. After evaporation to dryness, lipid conjugation was demonstrated by the extraction of the cluster into the chloroform layer when the palmitoyl cluster was shaken in a chloroform/water mixture with a small amount of methanol as a phase transfer agent.

1. customwas evaporated to dryness five times from methanol
2. dissolutiondissolved in 3 mL of diclhoromethane
3. washwashed three times with 0.1 M sodium phosphate buffer at pH 6.5
4. customThe remaining reaction mixture
5. customwas evaporated to dryness
6. dissolutionredissolved in 2:1 methanol/chloroform
7. customseparated by gel filtration (GH25, Amicon
8. washeluted with 2:1 methanol
9. washthe first species to elute
10. customAfter evaporation to dryness, lipid conjugation
11. extractionwas demonstrated by the extraction of the cluster into the chloroform layer when the palmitoyl cluster
12. stirringwas shaken in a chloroform/water mixture with a small amount of methanol as a phase transfer agent