Ammonia → L-Phenylalanine

Participants

Reaction Steps

Microbial cells (0.5 g) of Endomyces lindneri AJ-6611 (FERM P-7425, FERM BP-690), grown and washed by the same manner as Example 1, and microbial cells (0.5 g each) of AJ-117129 (FERM P-7737, FERM BP-693) and AJ-6307 (FERM P-7892, FERM BP-694), grown and washed in the same manner as Example 2, were each put in a dialysis membrane (0.6 cm in diameter; Visking Company), and the two ends were tightly closed by tying with strings, forming a bag containing the microbial cells. It was immersed in 10 ml of an aqueous solution containing 2 g/dl of cinnamic acid and 55 ml/dl of 28% ammonia (pH controlled to 10.5 with hydrochloric acid), and the reaction was continued at 30° C. for 24 hours for AJ-6611 and at 30° C. for 72 hours for the others. The amounts of phenylalanine formed in the reaction solution are listed in Table 4.

Provenance & Evidence