Ammonia → L-Phenylalanine

HRID 845014Notes

Reaction equation

HRID 845014 reaction equation
[C:1]([OH:11])(=[O:10])[CH:2]=[CH:3][C:4]1[CH:9]=[CH:8][CH:7]=[CH:6][CH:5]=1.[NH3:12]>>[NH2:12][C@H:2]([C:1]([OH:11])=[O:10])[CH2:3][C:4]1[CH:5]=[CH:6][CH:7]=[CH:8][CH:9]=1

Participants

3

Reactant 2

HCID 222AmmoniaHCID 87842-Propenoic acid, 3-phenyl-

Product 1

HCID 6140L-Phenylalanine

Procedure

Microbial cells (0.5 g) of Endomyces lindneri AJ-6611 (FERM P-7425, FERM BP-690), grown and washed by the same manner as Example 1, and microbial cells (0.5 g each) of AJ-117129 (FERM P-7737, FERM BP-693) and AJ-6307 (FERM P-7892, FERM BP-694), grown and washed in the same manner as Example 2, were each put in a dialysis membrane (0.6 cm in diameter; Visking Company), and the two ends were tightly closed by tying with strings, forming a bag containing the microbial cells. It was immersed in 10 ml of an aqueous solution containing 2 g/dl of cinnamic acid and 55 ml/dl of 28% ammonia (pH controlled to 10.5 with hydrochloric acid), and the reaction was continued at 30° C. for 24 hours for AJ-6611 and at 30° C. for 72 hours for the others. The amounts of phenylalanine formed in the reaction solution are listed in Table 4.

Workup

  1. washwashed by the same manner as Example 1, and microbial cells (0.5 g each) of AJ-117129 (FERM P-7737, FERM BP-693)
  2. washwashed in the same manner as Example 2
  3. customthe two ends were tightly closed
  4. customforming a bag
  5. additioncontaining the microbial cells
  6. waitfor AJ-6611 and at 30° C. for 72 hours for the others

Sources & evidence

ORD record
ord-487ab25f3e66448787f8c69d3a621121
Source dataset
uspto-grants-1988_07
Patent number
US04755466

Reaction notes

Loading…