L-Homoserine → L-Lysine

Participants

Reaction Steps

Plasmid pAJ 201 was introduced into Brevibacterium lactofermentum AJ 12019 (NRRL B-15346) (auxotrophy for homoserine) by the transformation method described in (4). The results obtained by examination of lysine productivity with the thus-obtained transformant AJ 12073 (FERM-P 7205) are shown in Table 3. The cultivation was carried out by charging in a shoulder-equipped flask 20 ml of medium containing 10 g/dl of glucose, 5.5 g/dl of ammonium sulfate, 0.1 g/dl of KH2PO4, 0.1 g/dl of MgSO4 . 0.001 g/dl of FeSO4 . 4H2O, 0,001 g/dl of MnSo4 . 4H2 0, 200 μg/l of thiamine hydrochloride, 2.5 mg/dl of nicotinamide, 105 mg/dl of soybean protein hydrolysate (as total nitrogen), and 5 g/dl of calcium carbonate, the pH of which had been adjusted to 8.0, while shaking at 31.5° C. for 72 hours. After completion of the cultivation, lysine in the culture liquid was quantitatively determined with high speed liquid chromatography.

Provenance & Evidence