Ethanol → HCID 13206015, compound
Reaction equation
[CH2:1]([O:3][C:4]([C@@H:6]([NH:15][C@H:16]([C:18](Cl)=[O:19])[CH3:17])[CH2:7][CH2:8][C:9]1[CH:14]=[CH:13][CH:12]=[CH:11][CH:10]=1)=[O:5])[CH3:2].[CH2:21]([OH:23])[CH3:22]>>[CH2:21]([O:23][C:18](=[O:19])[C@H:16]([CH3:17])[NH:15][C@H:6]([C:4]([O:3][CH2:1][CH3:2])=[O:5])[CH2:7][CH2:8][C:9]1[CH:14]=[CH:13][CH:12]=[CH:11][CH:10]=1)[CH3:22]Participants
Procedure
In the Examples, the purity of the obtained inorganic acid salt of N-[1(S)-ethoxycarbonyl-3-phenylpropyl]-L-alanylchloride was measured by reacting the obtained acid chloride with ethanol in the presence of a base to produce N-[1(S)-ethoxycarbonyl-3-phenylpropyl]-L-alanine ethylester, which was then subjected to a high performance liquid chromatography (hereinafter referred to as "HPLC"). More particularly, about 100 mg of the sample of the acid chloride was put in a 10 ml messflask, to which absolute ethanol containing 0.3% (w/v) of triethylamine was added to give 10 ml of the solution and the esterification was carried out by stirring the solution with a magnetic stirrer at room temperature for 30 minutes. To 1.0 ml of the sample of the thus obtained solution, 4 ml of ethanol solution containing 10 mg of n-propylbenzoic acid (internal standard reagent) was added, the mixture was subjected to the analysis by the column chromatography under the following conditions.
Sources & evidence
- ORD record
- ord-9338c70cc8db414ca283a9373b51c224
- Source dataset
- uspto-grants-1988_07
- Patent number
- US04760162
Reaction notes
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