Ethanol → HCID 13206015, compound

Participants

Reaction Steps

In the Examples, the purity of the obtained inorganic acid salt of N-[1(S)-ethoxycarbonyl-3-phenylpropyl]-L-alanylchloride was measured by reacting the obtained acid chloride with ethanol in the presence of a base to produce N-[1(S)-ethoxycarbonyl-3-phenylpropyl]-L-alanine ethylester, which was then subjected to a high performance liquid chromatography (hereinafter referred to as "HPLC"). More particularly, about 100 mg of the sample of the acid chloride was put in a 10 ml messflask, to which absolute ethanol containing 0.3% (w/v) of triethylamine was added to give 10 ml of the solution and the esterification was carried out by stirring the solution with a magnetic stirrer at room temperature for 30 minutes. To 1.0 ml of the sample of the thus obtained solution, 4 ml of ethanol solution containing 10 mg of n-propylbenzoic acid (internal standard reagent) was added, the mixture was subjected to the analysis by the column chromatography under the following conditions.

Provenance & Evidence