3-Cyanopyridine → Nicotinic Acid

Participants

Reaction Steps

Each of the cell suspensions was added in an amount of 0.1 ml to a reaction solution comprising 50 mM acrylonitrile or 1.0 ml of 3-cyanopyridine, 0.5 ml of a 100 mM potassium phosphate buffer pH 7.8 and 0.4 ml of distilled water, and the reaction was conducted at 20° C. for 20 minutes. The reaction was terminated by the addition of 0.2 ml of 1N HCl, and the amount of acrylic acid or nicotinic acid produced was assayed by HPLC wherein the M&S Pack Cl8 column manufactured by MS Kiki, Inc., Japan was used.

Provenance & Evidence