Butyric Acid → Formate

Participants

Reaction Steps

The reaction in Example 7 was repeated, using an aqueous solution containing glycolic acid (0.75M), aminomethylphosphonic acid (AMPA, 0.60M), FMN (0.01 mM), butyric acid (HPLC internal standard, 0.10M), glycolate oxidase (from spinach, 2.0 IU/mL), and catalase (from Aspergillus niger, 14,000 IU/mL) at pH 8.5.. After 40 h, the HPLC yields of glyoxylate, formate, and oxalate were 83.2%, 2.3%, and 7.5%, respectively, and no glycolate remained. The remaining activity of glycolate oxidase and catalase were 65% and 86%, respectively, of their initial values.

Provenance & Evidence