反应详情

A chromatography column containing a gel (Sephadex LH-20) 3 cm in diameter and 50 cm tall is prepared. 2.4 g of the impure extract obtained in step iii) diluted with ethanol is introduced into the column. The impure extract is eluted with ethanol at a rate of 2 ml per minute. A total of 17 fractions are obtained, 6 of these fractions containing the malonates of genistin and daidzin. These 6 fractions are subjected to a second chromatography in a column containing octadecyl silane groups attached to silica (Lobar RP-18). A 10% aqueous ethanol solution is used as the eluent at a rate of 2 ml per minute. Two fractions each containing a different product are finally obtained. These fractions are dried under reduced pressure in an evaporator at a temperature of 40° C. 25 mg daidzin malonate and 80 mg genistin malonate, both highly pure (92-98%), are obtained in the form of amorphous solids, the first white in colour and the second pale yellow in colour.

1. additioncontaining octadecyl silane groups
2. customTwo fractions each containing a different product are finally obtained
3. customThese fractions are dried under reduced pressure in an evaporator at a temperature of 40° C