反应详情

A mixture of 10 mg (0.033 mmol) of the resultant compound of Example 171 and 38.6 mg (0.10 mmol) of Cbz-isoleucine p-nitrophenyl ester in 0.2 ml of tetrahydrofuran was stirred at ambient temperature for 21 h. The resulting misture was diluted with 1 ml of tetrahydrofuran, treated with 0.5 ml of 3N NaOH, stirred for 45 min, extracted with chloroform, washed sequentially with 3N NaOH and saturated brine, dried over MgSO4, and concentrated. The residue was purified on silica gel by eluting with 2% methanol in chloroform to provide 23 mg (86%) of the desired compound. Mass spectrum: (M+H)+ =795.

1. stirringstirred for 45 min
2. extractionextracted with chloroform
3. washwashed sequentially with 3N NaOH and saturated brine
4. dry with materialdried over MgSO4
5. concentrationconcentrated
6. customThe residue was purified on silica gel
7. washby eluting with 2% methanol in chloroform