反应详情

温度

70 °C

N-[4-[2-(2-Amino-4,7-dihydro-4-oxo-1H-pyrrolo[2,3-d]pyrimidin-5-yl)ethyl]benzoyl]-L-glutamic acid from Example 7 was dissolved in 3.8 mL of water and 2.2 ml of 1N sodium hydroxide. The pH of the mixture was adjusted to 7.5-8.5 using dilute hydrochloric acid and 1N sodium hydroxide. The solution was heated to 70° C. and 40 mL of 3A alcohol were added. The solution was allowed to cool to room temperature during which time a thick slurry developed. The solids were filtered and washed with 4:1 ethanol:water. The solids were dried at 50° C. in a vacuum oven. 640 milligrams of the title compound were recovered as a solid.

1. temperatureto cool to room temperature during which time a thick slurry
2. filtrationThe solids were filtered
3. washwashed with 4:1 ethanol
4. customThe solids were dried at 50° C. in a vacuum oven
5. custom640 milligrams of the title compound were recovered as a solid