反应详情

Epicatechin (EC) (0.5 g) and gallic acid (1 g) were dissolved in a mixture of acetone-pH 5.0 phosphate citrate buffer (1: 10, v/v, 50 mL), which contained 2 mg horseradish peroxidase. While being stirred, 2.0 mL of 3.13% H2O2 was added four times during 45 minutes. The reaction mixture was extracted by ethyl acetate (50 mL×3). After concentration, the residue was subjected to Sephadex LH 20 column eluted with acetone-water solvent system (45%). 70 mg epitheaflavic acid and 25 mg purpurogallin carboxylic acid were obtained.

1. extractionThe reaction mixture was extracted by ethyl acetate (50 mL×3)
2. concentrationAfter concentration
3. washeluted with acetone-water solvent system (45%)