HRID853788

反应详情

EQUATION

反应方程式

REACTANTS

反应物

HCID280

Carbon dioxide

CO2

结构：CC1(C)S[C@@H]2[C@H](NC(=O)Cc3ccccc3)C(=O)N2[C@H]1C(=O)[O-].[K+]

HCID23664709

Penicillin G potassium

C16H17KN2O4S

结构：CN[C@@H]1[C@H](O[C@H]2[C@H](O[C@H]3[C@H](O)[C@@H](O)[C@H](NC(=N)N)[C@@H](O)[C@@H]3NC(=N)N)O[C@@H](C)[C@]2(O)C=O)O[C@@H](CO)[C@H](O)[C@H]1O

HCID178484872

未命名化合物

PRODUCTS

生成物

结构：CN1CC[C@]23c4c5ccc(O)c4O[C@H]2[C@@H](O)C=C[C@H]3[C@H]1C5

HCID5288826

Morphine

C17H19NO3

CONDITIONS

反应条件

温度: 37 °C

PROCEDURE

实验过程

Human neuroblastoma SH-SY5Y cells were maintained as a monolayer culture in Dulbecco's modified eagle medium (DMEM) supplemented with 10% fetal calf serum, 100 μg/ml streptomycin and 100 units/ml penicillin in 5% carbon dioxide, incubator maintained at 37° C. (Wang et al., 2000). Cells were plated into flasks a week before experiments Treatments were terminated at the designated times by replacing the medium with ice-cold phosphate buffered saline (PBS) on ice and subsequently rinsed with PBS three times. Cells then were used for cAMP assay or harvested as described in Section C10

WORKUP

后处理

temperatureHuman neuroblastoma SH-SY5Y cells were maintained as a monolayer culture in Dulbecco's
waitCells were plated into flasks a week before experiments Treatments
customwere terminated at the designated times
washsubsequently rinsed with PBS three times