Carbon Dioxide → Morphine

Participants

Reaction Conditions

Reaction Steps

Human neuroblastoma SH-SY5Y cells were maintained as a monolayer culture in Dulbecco's modified eagle medium (DMEM) supplemented with 10% fetal calf serum, 100 μg/ml streptomycin and 100 units/ml penicillin in 5% carbon dioxide, incubator maintained at 37° C. (Wang et al., 2000). Cells were plated into flasks a week before experiments Treatments were terminated at the designated times by replacing the medium with ice-cold phosphate buffered saline (PBS) on ice and subsequently rinsed with PBS three times. Cells then were used for cAMP assay or harvested as described in Section C10

Provenance & Evidence