反应详情
EQUATION
反应方程式
REACTANTS
反应物
Adenosine 5′-(trihydrogen diphosphate), 2′-(dihydrogen phosphate), P′→5′-ester with 3-(aminocarbonyl)-1-β-D-ribofuranosylpyridinium
C21H29N7O17P3+
未命名化合物
alpha-D-Glucose 1,6-bis(dihydrogen phosphate)
C6H14O12P2
Glutathione
C10H17N3O6S
Glucose 6-phosphate
C6H13O9P
Magnesium Chloride
Cl2Mg
PRODUCTS
生成物
AUXILIARIES
试剂、催化剂与溶剂
PROCEDURE
实验过程
The enzymic activity is determined according to the following procedure: 1.72 g of glutathione, and 34.2 mg of Na2EDTA are dissolved in a 114 mM potassium phosphate buffer solution (pH 7.0) to prepare 100 ml of a solution. A mixture of 700 μl of the solution, 700 μl of a thermally dissolved 5.72% starch solution, 100 μl of a 20 mM NADP+ solution, 100 μl of a 26 mM magnesium chloride solution, 100 μl of a 1.34 mM glucose 1,6-diphosphate solution, 100 μl of a 31 unit/ml phosphoglucomutase solution, 100 μl of a 35 unit/ml glucose 6-phosphate dehydrogenase solution, and 100 μl of a sample enzyme solution is incubated at 30° C. and the quantity of produced NADPH is monitored consecutively by means of absorption spectrophotometry at 340 nm. One unit refers to the amount of enzyme which is capable of producing 1 μ mol of NADPH for 1 min. under the conditions.
WORKUP
后处理
- customis incubated at 30° C.
- customthe quantity of produced NADPH is monitored consecutively by means of absorption spectrophotometry at 340 nm