NADP nicotinamide-adenine-dinucleotide phosphate → NADPH

Beteiligte Stoffe

Reaktionsschritte

The enzymic activity is determined according to the following procedure: 1.72 g of glutathione, and 34.2 mg of Na2EDTA are dissolved in a 114 mM potassium phosphate buffer solution (pH 7.0) to prepare 100 ml of a solution. A mixture of 700 μl of the solution, 700 μl of a thermally dissolved 5.72% starch solution, 100 μl of a 20 mM NADP+ solution, 100 μl of a 26 mM magnesium chloride solution, 100 μl of a 1.34 mM glucose 1,6-diphosphate solution, 100 μl of a 31 unit/ml phosphoglucomutase solution, 100 μl of a 35 unit/ml glucose 6-phosphate dehydrogenase solution, and 100 μl of a sample enzyme solution is incubated at 30° C. and the quantity of produced NADPH is monitored consecutively by means of absorption spectrophotometry at 340 nm. One unit refers to the amount of enzyme which is capable of producing 1 μ mol of NADPH for 1 min. under the conditions.

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