Water → Theophylline

HRID 865911Notes

Reaction equation

HRID 865911 reaction equation
C([C:4]1[NH:16][C:15]2[C:14](=[O:17])[N:9]([CH2:10]C(O)=O)[C:8](=[O:18])[N:7]([CH3:19])[C:6]=2[N:5]=1)CC.ON1C(=O)CCC1=O.O>CN(C=O)C>[N:9]1([C:14](=[O:17])[C:15]2[NH:16][CH:4]=[N:5][C:6]=2[N:7]([CH3:19])[C:8]1=[O:18])[CH3:10]

Participants

5

Reactant 3

HCID 962WaterHCID 80170N-HydroxysuccinimideHCID 86745509HCID 86745509, compound

Product 1

HCID 2153Theophylline

Reagents, catalysts & solvents 1

HCID 6228DimethylformamideSolvent

Procedure

5 mg of 8-propylcarboxytheophylline was dissolved in 1 ml of DMF, and added with 3 mg of N-hydroxysuccinimide and 5 mg of water-soluble carbodiimide, followed by stirring at room temperature for 2 hours, to obtain activated theophylline. 10 mg of bovine serum albumin (BSA) was dissolved in 1 ml of a 0.1M aqueous solution of sodium hydrogencarbonate and added with 500 μl of the aforementioned activated theophylline solution. The mixed solution was allowed to stand at room temperature for an hour, and then unreacted substances were removed through a SEPHADEX G-25 (produced by Pharmacia Co.) column preliminarily equilibrated with PBS to obtain 9 mg of the objective large molecule antigen (theophylline-BSA conjugate)

Sources & evidence

ORD record
ord-6ab443e685a14264bb6ef5a296848ab1
Source dataset
uspto-grants-1996_10
Patent number
US05569589

Reaction notes

Loading…