反应详情

A solution of DL-2-piperidinecarbonyl-α-L-aspartyl-L-phenylalanine methyl ester hydrochloride (2.1 g) in 50 ml water was neutralized with sodium bicarbonate and passed through a column (50 ml) packed with an adsorption resin of styrene/divinylbenzene type (Mitsubishi Chemical Industries, Ltd., "Adsorption Resin SP-207"). After washing the column with 200 ml water, the adsorbed portion was eluted with 200 ml of water/methanol (50/50 by volume), and the solvents were removed by distillation, affording 1.7 g of white powder M.p.: 225°-227° C.

1. washAfter washing the column with 200 ml water
2. washthe adsorbed portion was eluted with 200 ml of water/methanol (50/50 by volume)
3. customthe solvents were removed by distillation