HRID905529

反应详情

EQUATION

反应方程式

REACTANTS

反应物

结构：O=c1ccn([C@@H]2O[C@H](CO)[C@@H](O)[C@H]2O)c(=O)[nH]1

HCID6029

Uridine

C9H12N2O6

结构：O=c1ccn([C@H]2C[C@H](O)[C@@H](CO)O2)c(=O)[nH]1

HCID13712

Deoxyuridine

C9H12N2O5

HCID94904

2-Chloroadenine

C5H4ClN5

PRODUCTS

生成物

结构：Nc1nc(Cl)nc2c1ncn2[C@H]1C[C@H](O)[C@@H](CO)O1

HCID20279

Cladribine

C10H12ClN5O3

PROCEDURE

实验过程

reaction of 2-deoxyuridine with 2-chloroadenine, in the presence of uridine phosphorylase (UPase) and purine nucleoside phosphorylase (PNPase) in an aqueous reaction medium possibly containing up to 40% v/v of an aprotic dipolar solvent, to obtain cladribine dissolved in said reaction medium; b) isolation of the cladribine by precipitation by means of concentration and alkalinisation of the reaction medium up to a pH of 11.5-12.5.