反应详情

To a suspension of lithium aluminum hydride (150 mg, 4.0 mmol) in THF (15 ml) was added a solution of 2-butoxy-9-[2-(3-methoxycarbonylphenoxy)ethyl]adenine (540 mg, 1.4 mmol) obtained in Reference Example 2 in THF (5 ml) under ice-cooling, and the mixture was stirred at room temperature for 3 hours. 1N Aqueous sodium hydroxide solution was added thereto, followed by filtration through celite pad and concentration under reduced pressure. The residue was dissolved in chloroform (10 ml), and thionyl chloride (336 μl, 4.62 mmol) was added thereto at room temperature, followed by stirring at 60° C. for 10 minutes. To the reaction mixture was added 1N aqueous sodium hydroxide solution, and the mixture was diluted with water and extracted with chloroform (methanol 5%). The organic layer was washed with water and brine, dried over sodium sulfate and concentrated under reduced pressure to give chloromethyl product as a pale yellow crystal, 380 mg. To a solution of the chloromethyl product (380 mg) in DMF (10 ml) was added sodium cyanide (99 mg, 2.02 mmol) at room temperature, followed by stirring at room temperature for 1 hour. To the reaction mixture was added saturated ammonium chloride and the mixture was concentrated under reduced pressure. To the residue was added water and the mixture was extracted with chloroform (methanol 5%). The organic layer was washed with water and saturated brine, dried over sodium sulfate and concentrated under reduced pressure. To the residue were added methanol (7 ml) and 5N aqueous potassium hydroxide solution (7 ml) and the mixture was stirred at 95° C. for 3.5 hours. The reaction solution was neutralized with concentrated hydrochloric acid, and concentrated under reduced pressure. Methanol (20 ml) and concentrated sulfuric acid (0.3 ml) were added thereto, followed by stirring at 90° C. for 3.5 hours. After cooling, the resultant was concentrated under reduced pressure, and to the residue was added water. The solution was extracted with chloroform (methanol 5%). The organic layer was washed with water and saturated brine, dried over sodium sulfate and concentrated under reduced pressure. The residue was purified by silica gel column chromatography to give 160 mg (0.40 mmol) of the titled compound as a pale yellow oil. Yield: 29%.

1. temperaturecooling
2. filtrationfollowed by filtration through celite pad and concentration under reduced pressure
3. dissolutionThe residue was dissolved in chloroform (10 ml)
4. customat room temperature
5. stirringby stirring at 60° C. for 10 minutes
6. extractionextracted with chloroform (methanol 5%)
7. washThe organic layer was washed with water and brine
8. dry with materialdried over sodium sulfate
9. concentrationconcentrated under reduced pressure
10. customto give chloromethyl product as a pale yellow crystal, 380 mg
11. stirringby stirring at room temperature for 1 hour
12. concentrationthe mixture was concentrated under reduced pressure
13. additionTo the residue was added water
14. extractionthe mixture was extracted with chloroform (methanol 5%)
15. washThe organic layer was washed with water and saturated brine
16. dry with materialdried over sodium sulfate
17. concentrationconcentrated under reduced pressure
18. additionTo the residue were added methanol (7 ml) and 5N aqueous potassium hydroxide solution (7 ml)
19. stirringthe mixture was stirred at 95° C. for 3.5 hours
20. concentrationconcentrated under reduced pressure
21. additionMethanol (20 ml) and concentrated sulfuric acid (0.3 ml) were added
22. stirringby stirring at 90° C. for 3.5 hours
23. temperatureAfter cooling
24. concentrationthe resultant was concentrated under reduced pressure
25. additionto the residue was added water
26. extractionThe solution was extracted with chloroform (methanol 5%)
27. washThe organic layer was washed with water and saturated brine
28. dry with materialdried over sodium sulfate
29. concentrationconcentrated under reduced pressure
30. customThe residue was purified by silica gel column chromatography